分子克隆实验指南 : 第4版

VOLUME 1

Part 1： Essentials

1. Isolation and Quantification of DNA

2. Analysis of DNA

3. Cloning and Transformation with Plasmid Vectors

4. Gateway Recombinational Cloning

John S. Reece-Hoyes and Albertha J.M. Walhout

5. Working with Bacterial Artificial Chromosomes and OtherHigh-Capacity Vectors

Nathaniel Heintz and Shiaoching Gong

6. Extraction， Purification， and Analysis of RNA from EukaryoticCells

7. Polymerase Chain Reaction

8. Bioinformatics

Jui-Hung Hung and Zhiping Weng

VOLUME 2

Part 2： Analysis and Manipulation of DNA and RNA

9. Quantification of DNA and RNA by Real-Time Polymerase ChainReaction

10. Nucleic Acid Platform Technologies

Oliver Rando

11. DNA Sequencing

Elaine Mardis and W. Richard McCombie

12. Analysis of DNA Methylation in Mammalian Cells

Paul M. Lizardi， Qin Yan， and Narendra Wajapeyee

13. Preparation of Labeled DNA， RNA， and OligonucleotideProbes

14. Methods for In Vitro Mutagenesis

Matteo Forloni， Alex Liu， and Narendra Wajapeyee

Part 3： Introducing Genes into Cells

15. Introducing Genes into Cultured Mammalian Cells

Priti Kumar， Arvindhan Nagarajan， and Pradeep D. Uchil

16. Introducing Genes into Mammalian Cells： Viral Vectors

Guangping Gao and Miguel Sena-Esteves

VOLUME 3

Part 4： Gene Expression

17. Analysis of Gene Regulation Using Reporter Systems

Pradeep D. Uchil， Arvindhan Nagarajan， and Priti Kumar

18. RNA Interference and Small RNA Analysis

Chengjian Li and Phillip D. Zamore

19. Expressing Cloned Genes for Protein Production， Purification，and Analysis

Clara L. Kielkopf， William Bauer， and Ina Urbatsch

Part 5： Interaction Analysis

20. Cross-Linking Technologies for Analysis of Chromatin Structureand Function

Tae Hoon Kim and Job Dekker

21. Mapping of In Vivo RNA-Binding Sites by UV-Cross-LinkingImmunoprecipitation （CLIP）

Jennifer C. Darnell， Aldo Mele， Ka Ying Sharon Hung， and Robert B.Darnell

22. Gateway-Compatible Yeast One-Hybrid and Two-Hybrid Assays

John S. Reece-Hoyes and Albertha J.M. Walhout

Appendices

1. Reagents and Buffers

2. Commonly Used Techniques

3. Detection Systems

4. General Safety and Hazardous Material

Molecular Cloning has served as the foundation of technicalexpertise in labs worldwide for 30 years. No other manual has beenso popular， or so influential. Molecular Cloning， Fourth Edition，by the celebrated founding author Joe Sambrook and new co-author，the distinguished HHMI investigator Michael Green， preserves thehighly praised detail and clarity of previous editions and includesspecific chapters and protocols commissioned for the book fromexpert practitioners at Yale， U Mass， Rockefeller University， TexasTech， Cold Spring Harbor Laboratory， Washington University， andother leading institutions. The theoretical and historicalunderpinnings of techniques are prominent features of thepresentation throughout， information that does much to helptrouble-shoot experimental problems.

　　For the fourth edition of this classic work， the content hasbeen entirely recast to include nucleic-acid based methods selectedas the most widely used and valuable in molecular and cellularbiology laboratories.

　　Core chapters from the third edition have been revised tofeature current strategies and approaches to the preparation andcloning of nucleic acids， gene transfer， and expression analysis.They are augmented by 12 new chapters which show how DNA， RNA， andproteins should be prepared， evaluated， and manipulated， and howdata generation and analysis can be handled.

　　The new content includes methods for studying interactionsbetween cellular components， such as microarrays， next-generationsequencing technologies， RNA interference， and epigenetic analysisusing DNA methylation techniques and chromatin immunoprecipitation.To make sense of the wealth of data produced by these techniques， abioinformatics chapter describes the use of analytical tools forcomparing sequences of genes and proteins and identifying commonexpression patterns among sets of genes.

　　Building on thirty years of trust， reliability， and authority，the fourth edition of Molecular Cloning is the new goldstandard—the one indispensable molecular biology laboratory manualand reference source.